Protocol for evaluation of teat cleaning systems

Deliverable D13

Authors: K. Knappstein, N. Roth, B.A. Slaghuis, R.T. Ferweda-van Zonneveld

The cleaning of udder and teats before milking contributes to the image of hygienic food production. Teat and udder surfaces belong to the main sources of bacteria in raw milk. Therefore appropriate cleaning procedures are necessary to reduce the microbial contamination of raw milk. As part of workpackage 6 within the European project "Implications of the Introduction of Automatic Milking on Dairy Farms" a protocol was developed to evaluate mechanised teat cleaning devices that are in operation in automatic milking systems.
The evaluation of teat cleaning procedures will be based on a combination of four methods: visual inspection, sediment test on teat swabs and determination of total bacterial counts (TBC) and ATP in teat swabs. The results of visual inspection are largely dependent on the evaluating person and also vary within testing person when the same material is evaluated repeatedly. Nevertheless, visual inspection and sediment tests are suitable to determine teat cleanliness if cleanliness is defined as the absence of visible contamination with dirt and/or manure. For cleaned teats identical scoring by the two methods was determined in 95.8 % (n=96). The total impression of udder cleanliness seemed to be mainly influenced by the status of the udder basis.
By comparison of TBC and ATP in teat swabs taken before and after teat cleaning a differentiation of cleaning efficiency of manual teat cleaning methods was possible. By wet cleaning with subsequent drying a better teat cleaning effect was achieved: the reduction of TBC in Log10 units was 1.50 (wet cleaning with subsequent drying of the udder) versus 1.11 (dry cleaning), the reduction of ATP in Log10 RLU was 1.26 versus 0.56. The efficiency of manual cleaning methods will serve as a reference for the following evaluation of mechanised cleaning devices applied in practice during the second part of the study.
Another approach for the evaluation of teat cleaning efficiency was based on artificial contamination of teats with cobalt and subsequent determination of its carry over into milk, but could not be validated yet. The loss of cobalt after application on teats was high. Although carry over from teats and from teat cleaning devices into milk was found, no difference between cleaning and no cleaning of teats could be determined when the method was applied on an automatic milking system. Additional experiments are necessary to validate the cobalt method.

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