Effectiveness of optimised teat cup cleaning in the prevention of mastitis pathogens transfer

Deliverable D18

Authors: H.J. Schuiling, F. Neijenhuis

Flushing of the teatcup liners between the milkings of two cows is a common procedure in AMsystems.
In this investigation the standard procedure, flushing with cold water, is compared with flushing with a disinfecting fluid, composed of cold water and per acetic acid. Liners were contaminated with a suspension of Streptococcus agalactiae in milk, comparable with a severe inflammation of the udder. The log reduction in number of pathogens was 1,80 and 2,26 for respectively cold water and the disinfectant. The extra log reduction with a disinfectant was small and did not compensate for the negative effects of using disinfectants in a milking system (extra rinse necessary, higher risk of contamination of milk).
Further experiments to show the effect of cluster flush on transfer of pathogens through liners are done with a cold water rinse only.

46 Cows were challenged with pathogens by milking with deliberately contaminated liners with a high dose of Streptococcus agalactiae prior to the cluster flush. Two liners (cross positioned front and rear) were flushed after contamination, the other two liners were not flushed. The udder health of the cows was measured during one week after the challenge. In none of the foremilk samples Streptococcus agalactiae could be recovered and none of quarters showed signs of an inflammation (increase in SCC or conductivity). The effect of a cluster flush on the rate of new infections could not be established in this experiment.
However, based on the results of the reduction of pathogens in liners, it is recommended to perform a cluster flush to reduce the number of pathogens in the liner.

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